ahkit81 Posted May 25, 2004 Share Posted May 25, 2004 heiya im new in this forum hope you guys will help me out. I plan to setup a phytoplankton culture. But i cant able to find the culturing medium over at malaysia. Is DT able to replace this? Read from the forum here look like it is possible. Another question is i'm unable to find the florida farms fertiliser in malaysia . is any any other stuffs can be replace the fertiliser used for culturing this phytoplankton? Hope you guys can help me! Thanx Quote Link to comment Share on other sites More sharing options...
RockyBoy Posted May 25, 2004 Share Posted May 25, 2004 You can order from florida farm directly. I've done that before and its ok. Or you can make a trip to singapore, any one of us will be happy to bring you to those shops selling phyto culture stuffs. Quote Member of : UEN: T08SS0098FMASS in Facebook Reefing in LED Link to comment Share on other sites More sharing options...
ahkit81 Posted May 25, 2004 Author Share Posted May 25, 2004 Been there last month visited R####N and another one near orchard road. Cool stuffs you guys got over there. Didn't get the starter kit from R####N coz it look kinda old stock scared it doesn't works hehe. As for the florida fertiliser didn't manage to find it Quote Link to comment Share on other sites More sharing options...
SRC Member ervine Posted May 25, 2004 SRC Member Share Posted May 25, 2004 Been there last month visited R####N and another one near orchard road. Cool stuffs you guys got over there. Didn't get the starter kit from R####N coz it look kinda old stock scared it doesn't works hehe. As for the florida fertiliser didn't manage to find it Reborn sells the ferts... but the culture likewise looked a little... erm... unhealthy... Anyone know's the chemical composition of the ferts? Can probably mix up a similar batch... Quote Link to comment Share on other sites More sharing options...
ahkit81 Posted May 27, 2004 Author Share Posted May 27, 2004 yeah anyone know??any idea what the fertilizer contain?? maybe i can mix myself or do a cocktail myself Quote Link to comment Share on other sites More sharing options...
RockyBoy Posted June 8, 2004 Share Posted June 8, 2004 Better dun go DIY your own fert. Would have undesirable consequences if you start dosing the phytos into your main tank. Quote Member of : UEN: T08SS0098FMASS in Facebook Reefing in LED Link to comment Share on other sites More sharing options...
ahkit81 Posted July 22, 2004 Author Share Posted July 22, 2004 just wondering can i use DT as a medium to start my culture?? or i should go get the strain......btw does anyone have the name for the strain??? i hope i can get these from those company that provide equipment for science lab Quote Link to comment Share on other sites More sharing options...
SRC Member bawater Posted July 22, 2004 SRC Member Share Posted July 22, 2004 There are 26 recipes for culture mediums - but the most common used are the L1,K and f/2 formulas. Guillard's f/2 formula (florida farms) version-Guillard & Ryther 1962, Guillard 1975 To 950 mL filtered seawater add: Quantity - Compound - Stock Solution - Molar Concentration in Final Medium 1 mL - NaNO3 - 75 g/L dH2O - 8.83 x 10-4 M 1 mL -NaH2PO4 · H2O - 5 g/L dH2O - 3.63 x 10-5 M 1 mL * -Na2SiO3 · 9H2O* - 30 g/L dH2O* - 1.07 x 10-4 M* 1 mL - f/2 trace metal solution 0.5 mL - f/2 vitamin solution If u are looking for a pure strain - the best & robust one will be Nannochlorpsis Oculata - cell size 2 microns. *Nannochlorpsis a unicellular microalgae, member of the Chromophyta phylum, class Eustigmatophyceae, with an average size of between 2-4 µm. Quote Link to comment Share on other sites More sharing options...
ahkit81 Posted July 23, 2004 Author Share Posted July 23, 2004 WOW...sound complicated.... im not a science guy..hehe...but do you think i can used sea water as my main source of pytoplankton...... then added in those fertiliser that is used for those fresh water planted tank? anyone tried it before? Quote Link to comment Share on other sites More sharing options...
RockyBoy Posted July 28, 2004 Share Posted July 28, 2004 May not a good idea to use seawater as phyto source. Seawater might contain other nasty stuffs such as bacteria,etc. Also can't use the normal plant fertiliser. You might indirectly add more phospate in your aquarium. Quote Member of : UEN: T08SS0098FMASS in Facebook Reefing in LED Link to comment Share on other sites More sharing options...
SRC Member Ahku Posted February 18, 2019 SRC Member Share Posted February 18, 2019 any idea where to buy Guillard's f/2 formula ? Quote Link to comment Share on other sites More sharing options...
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